i) The physiological process

The culture environment in which the very first stages of embryo development take place is critical.

For in vitro techniques the culture media remains artificial, static and often omits a large number of components that are naturally present in the uterine cavity. More importantly, the laboratory context misses all the biological interactions between the developing embryos and the maternal environment.


To date, there are a growing number of studies in Humans investigating the possible impact of culture conditions on embryo development and on various observed adverse perinatal outcomes (i.e. homeostasis, architecture of the mitotic spindle, birth weight, vascular function, diabetes, epigenetic control of the gene expression). Although there are some controversies and possible confounding factors; several published data show that some developmental issues and adverse outcomes occur more frequently after ART than with natural conception.

By minimizing the impact of artificial culture conditions (e.g., synthetic culture media) on the development of the embryo, the risk of alteration of the embryo by exogenous factors or the absence of important molecules for the regulation of the embryo development should be dramatically reduced.

It is therefore paramount to try to replicate in the traditional laboratory of a fertility centre the physiological composition of the natural maternal environment.

Anecova’s innovative approach places injected or inseminated eggs in the maternal uterine cavity during fertilization and very early development stages, so that the embryo can benefit from the mother’s natural, physiological environment.

Restoring fertilization and early development of the embryos to the maternal womb is expected to optimize the production of highly competent embryos. The embryos can benefit from the full range of nutrients and active substances in the maternal environment as well as signals from the mother.

This natural incubation procedure enables the future parents to play a more active role at the very beginning of fertility treatment. It allows a stronger emotional involvement in these early stages whilst the maternal environment responds to the presence of the embryos and is prepared for embryo implantation.


Zhu, Qiao, 2014, Hum Reprod

Taylor, Griffin, 2014, Hum Reprod

Kleijkers, Dumoulin, 2014, Hum Reprod

Pinborg, Bergh, 2013 Hum Reprod Update

Nelissen, van Montfoort, 2013, Hum Reprod

Nelissen, Dumoulin, 2013, Hum Reprod

Sherrer, Sartori, 2012, Circulation AHA

Chen, Heilbtonn, 2014, Diabetes   

Nelissen, Dumoulin, 2012, Hum Reprod

Vergouw, Schats, 2012, Hum Reprod

Bedaiwy, Agarwal, 2010, Fertil Steril